nucleosome substrate Search Results


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biotinylated epicypher  (EpiCypher)
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Figure 4. ARID1A IDRs mediate local proximity of cBAF complex with cellular transcriptional machinery, enabling ARID domain-dependent TF binding (A) Immunoblot for input and anti-HA IP from AN3CA cells expressing HA-ARID1A fused to biotin ligase TurboID (TbID). (B) Distribution of <t>biotinylated</t> proteins fold changes. (C) Volcano plots comparing biotinylated protein levels. (D) Immunofluorescence analysis of ARID1A and p300 in AN3CA cells. (E) Volcano plots comparing detected protein levels following IP-MS. (F) Overlap of ARID1A WT-carrying cBAF interactomes measured using proximity labeling or IP-MS. (G) Protein class enrichment of detected cBAF-interacting proteins via IP-MS (DNA interactors in red). (H) Input and selected transcription factor (cJUN, NFIA, TEAD1) reciprocal IPs using AN3CA cells expressing empty vector or WT- and mutant-ARID1A. See also Figure S4 and Table S2.
Biotinylated Epicypher, supplied by EpiCypher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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n 66  (EpiCypher)
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Figure 4. ARID1A IDRs mediate local proximity of cBAF complex with cellular transcriptional machinery, enabling ARID domain-dependent TF binding (A) Immunoblot for input and anti-HA IP from AN3CA cells expressing HA-ARID1A fused to biotin ligase TurboID (TbID). (B) Distribution of <t>biotinylated</t> proteins fold changes. (C) Volcano plots comparing biotinylated protein levels. (D) Immunofluorescence analysis of ARID1A and p300 in AN3CA cells. (E) Volcano plots comparing detected protein levels following IP-MS. (F) Overlap of ARID1A WT-carrying cBAF interactomes measured using proximity labeling or IP-MS. (G) Protein class enrichment of detected cBAF-interacting proteins via IP-MS (DNA interactors in red). (H) Input and selected transcription factor (cJUN, NFIA, TEAD1) reciprocal IPs using AN3CA cells expressing empty vector or WT- and mutant-ARID1A. See also Figure S4 and Table S2.
N 66, supplied by EpiCypher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/n 66/product/EpiCypher
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epidyne nucleosome remodeling assay substrate st601 gatc1  (EpiCypher)
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EpiCypher epidyne nucleosome remodeling assay substrate st601 gatc1
Figure 4. ARID1A IDRs mediate local proximity of cBAF complex with cellular transcriptional machinery, enabling ARID domain-dependent TF binding (A) Immunoblot for input and anti-HA IP from AN3CA cells expressing HA-ARID1A fused to biotin ligase TurboID (TbID). (B) Distribution of <t>biotinylated</t> proteins fold changes. (C) Volcano plots comparing biotinylated protein levels. (D) Immunofluorescence analysis of ARID1A and p300 in AN3CA cells. (E) Volcano plots comparing detected protein levels following IP-MS. (F) Overlap of ARID1A WT-carrying cBAF interactomes measured using proximity labeling or IP-MS. (G) Protein class enrichment of detected cBAF-interacting proteins via IP-MS (DNA interactors in red). (H) Input and selected transcription factor (cJUN, NFIA, TEAD1) reciprocal IPs using AN3CA cells expressing empty vector or WT- and mutant-ARID1A. See also Figure S4 and Table S2.
Epidyne Nucleosome Remodeling Assay Substrate St601 Gatc1, supplied by EpiCypher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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epidyne biotinylated nucleosome remodeling substrate  (EpiCypher)
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EpiCypher epidyne biotinylated nucleosome remodeling substrate
Figure 4. ARID1A IDRs mediate local proximity of cBAF complex with cellular transcriptional machinery, enabling ARID domain-dependent TF binding (A) Immunoblot for input and anti-HA IP from AN3CA cells expressing HA-ARID1A fused to biotin ligase TurboID (TbID). (B) Distribution of <t>biotinylated</t> proteins fold changes. (C) Volcano plots comparing biotinylated protein levels. (D) Immunofluorescence analysis of ARID1A and p300 in AN3CA cells. (E) Volcano plots comparing detected protein levels following IP-MS. (F) Overlap of ARID1A WT-carrying cBAF interactomes measured using proximity labeling or IP-MS. (G) Protein class enrichment of detected cBAF-interacting proteins via IP-MS (DNA interactors in red). (H) Input and selected transcription factor (cJUN, NFIA, TEAD1) reciprocal IPs using AN3CA cells expressing empty vector or WT- and mutant-ARID1A. See also Figure S4 and Table S2.
Epidyne Biotinylated Nucleosome Remodeling Substrate, supplied by EpiCypher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/epidyne biotinylated nucleosome remodeling substrate/product/EpiCypher
Average 93 stars, based on 1 article reviews
epidyne biotinylated nucleosome remodeling substrate - by Bioz Stars, 2026-02
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Figure 4. ARID1A IDRs mediate local proximity of cBAF complex with cellular transcriptional machinery, enabling ARID domain-dependent TF binding (A) Immunoblot for input and anti-HA IP from AN3CA cells expressing HA-ARID1A fused to biotin ligase TurboID (TbID). (B) Distribution of biotinylated proteins fold changes. (C) Volcano plots comparing biotinylated protein levels. (D) Immunofluorescence analysis of ARID1A and p300 in AN3CA cells. (E) Volcano plots comparing detected protein levels following IP-MS. (F) Overlap of ARID1A WT-carrying cBAF interactomes measured using proximity labeling or IP-MS. (G) Protein class enrichment of detected cBAF-interacting proteins via IP-MS (DNA interactors in red). (H) Input and selected transcription factor (cJUN, NFIA, TEAD1) reciprocal IPs using AN3CA cells expressing empty vector or WT- and mutant-ARID1A. See also Figure S4 and Table S2.

Journal: Cell

Article Title: A disordered region controls cBAF activity via condensation and partner recruitment.

doi: 10.1016/j.cell.2023.08.032

Figure Lengend Snippet: Figure 4. ARID1A IDRs mediate local proximity of cBAF complex with cellular transcriptional machinery, enabling ARID domain-dependent TF binding (A) Immunoblot for input and anti-HA IP from AN3CA cells expressing HA-ARID1A fused to biotin ligase TurboID (TbID). (B) Distribution of biotinylated proteins fold changes. (C) Volcano plots comparing biotinylated protein levels. (D) Immunofluorescence analysis of ARID1A and p300 in AN3CA cells. (E) Volcano plots comparing detected protein levels following IP-MS. (F) Overlap of ARID1A WT-carrying cBAF interactomes measured using proximity labeling or IP-MS. (G) Protein class enrichment of detected cBAF-interacting proteins via IP-MS (DNA interactors in red). (H) Input and selected transcription factor (cJUN, NFIA, TEAD1) reciprocal IPs using AN3CA cells expressing empty vector or WT- and mutant-ARID1A. See also Figure S4 and Table S2.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins GST-tagged ARID domain WT This study N/A GST-tagged ARID domain DBDmut This study N/A Blasticidin S HCl ThermoFisher Cat#R21001 Puromycin dihydrochloride Sigma-Aldrich Cat#P8833-25MG Doxycycline (hyclate) Cayman Chemical Cat#14422 Carbenecillin disodium Gold Bio Cat#C-103-5 Chloramphenicol Gold Bio Cat#C-105-5 TURBO DNase I ThermoFisher Cat#AM2238 Tn5 transposase Illumina Cat#20034198 EpiDyne Nucleosome Remodeling Assay Substrate ST601-GATC1, 50-N-66, Biotinylated Epicypher Cat#16-4114 HeLa Polynucleosomes, purified Epicypher Cat#16-0003 Biomag Plus Concanavalin A (ConA) magnetic beads Polysciences Cat#86057-3 CUTANA pAG-Tn5 Epicypher Cat#15-1117 CUTANA pAG-MNase Epicypher Cat#15-1116 DpnII restriction enzyme New England Biolabs Cat#R0543S Recombinant Proteinase K Solution (20mg/mL) ThermoFisher Cat#AM2546 Magnetic Streptavidin beads ThermoFisher Cat#88817 Protein G Dynabeads ThermoFisher Cat#10004D Pierce Anti-HA Magnetic Beads ThermoFisher Cat#88837 Critical commercial assays NextSeq 500/550 High Output Kit v2.5 (75 Cycles) Illumina Cat#20024906 NEBNext Ultra II RNA Library Prep Kit for Illumina New England Biolabs Cat#E7770L CUTANA CUT&RUN Library Prep Kit Epicypher Cat#14-1001 MinElute Reaction Cleanup Kit Qiagen Cat#28206 ADP-Glo Max Assay Promega Cat#V7001 BCA Protein Assay Kit ThermoFisher Cat#23225 SilverQuest Silver Staining Kit ThermoFisher Cat#LC6070 Pierce High pH Reversed-Phase Peptide Fractionation Kit ThermoFisher Cat#84868 TMTpro 16plex Label Reagent Set ThermoFisher Cat#A44520 Protein Qubit ThermoFisher Cat#Q33212 Deposited data All AN3CACUT&Tag, CUT&RUN, ATAC and RNA-Seq data This study GEO: GSE209961 Experimental models: Cell lines HEK293T DARID1A/B Mashtalir et al.19 N/A U2OS ATCC Cat#HTB-96; RRID: CVCL_0042 AN3CA ATCC Cat#HTB-111; RRID: CVCL_0028 MDA-MB-231 ATCC Cat#HTB-26; RRID: CVCL_0062 KLE ATCC Cat#CRL-1622; RRID: CVCL_1329 C2C12 ATCC Cat#CRL-1772; RRID: CVCL_0188 CRL-7250 ATCC Cat#CRL-7250, discontinued; RRID: CVCL_N613 (Continued on next page) ll OPEN ACCESS Cell 186, 4936–4955.e1–e13, October 26, 2023 e2 Article

Techniques: Binding Assay, Western Blot, Expressing, Protein-Protein interactions, Labeling, Plasmid Preparation, Mutagenesis